This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/dele...
This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and bar...
This book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on...
This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular charac...
Flow of ions through voltage gated channels can be represented theoretically using stochastic differential equations where the gating mechanism is represented by a Markov model. The flow through a channel can be manipulated using various drugs, and the effect of a given drug can be reflected by changing the Markov model. These lecture notes provide...